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KMID : 0616120210520020069
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Korean Journal of Pharmacognosy
2021 Volume.52 No. 2 p.69 ~ p.76
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Cannabinol Synthase Gene Based Molecular Markers for Identification of Drug and Fiber Type Cannabis sativa
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Park Hyun-Seung
Oh Hye-Hyun
Kim Sung-Min
Park Jee-Young
Kim Jin-Tae
Shim Hyeon-Ah
Yang Tae-Jin
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Abstract
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Cannabis sativa is an important industrial plant utilized to produce fiber, oil, and medicinal ingredients. A chemotype of cannabis is divided into “Drug type” with predominance of tetrahydrocannabinolic acid (THCA) and “Fiber type” with cannabidiolic acid (CBDA). To develop molecular markers for the discrimination of these two types, nucleotide sequences of THCA synthase and CBDA synthase as well as their pseudogenes were retrieved from the recently published cannabis genome in chromosome scale. Gene-specific SNPs were discovered by multiple alignment of these sequences, and 2 dominant marker sets from each gene were designed for selective amplification. Our markers successfully identified “Drug type” and “Fiber type” cannabis plants as well as forensic samples including processed materials. Our molecular markers will provide a fast and efficient system for molecular-based identification of the cannabis plant.
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KEYWORD
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Cannabis sativa, THCAS, CBDAS, Molecular marker
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